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Qian Baoying, Huang Hongli, Li Juan, Cao Mingyue, Zhang Yu, Zhao Yayun, Xue Liangyi. Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea[J]. Haiyang Xuebao, 2018, 40(12): 31-39

Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea

Qian Baoying1,2, Huang Hongli1, Li Juan1, Cao Mingyue1, Zhang Yu1, Zhao Yayun1, Xue Liangyi1,3
1. College of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou 317000, China;
3. Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315211, China
Hormone sensitive lipase (HSL) and acetyl-CoA carboxylase (ACC) are key enzymes in lipid metabolism. Muscle and liver tissues are major main places. To study the role of the two enzymes during fasting, we detected the mRNA expression and activity changes of HSL and ACC in large yellow croaker muscle and liver tissues during 35 d fasting by real-time fluorescent quantitative PCR (qPCR) and Elisa, respectively. The results showed that fasting significantly decreased fat content and ACC mRNA level (P< 0.05), and increased HSL mRNA expression in both muscle and liver tissues (P<0.05). Moreover, fasting had a significant influence on the activities of HSL and ACC in both muscle and liver tissues of large yellow croaker (P<0.05). Muscle and liver HSL activities were moderately correlated with the corresponding mRNA expression level, as well as ACC in muscle. But liver ACC activity was negatively correlated with the mRNA level. These results suggested that the expression of HSL and ACC were regulated in both pre-translational and post-translational levels during fasting in the large yellow croaker. The results of the present study provide insights into the molecular mechanisms underlying the lipid metabolic response of the large yellow croaker to fasting.
Key words:    Larimichthys crocea    hormone sensitive lipase    acetyl-CoA carboxylase    fasting    mRNA expression    enzymatic activity   
Received: 2017-12-11   Revised: 2018-01-18
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