Cite this paper:
Qian Baoying, Huang Hongli, Li Juan, Cao Mingyue, Zhang Yu, Zhao Yayun, Xue Liangyi. Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea[J]. Haiyang Xuebao, 2018, 40(12): 31-39

Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea

Qian Baoying1,2, Huang Hongli1, Li Juan1, Cao Mingyue1, Zhang Yu1, Zhao Yayun1, Xue Liangyi1,3
1. College of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou 317000, China;
3. Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315211, China
Abstract:
Hormone sensitive lipase (HSL) and acetyl-CoA carboxylase (ACC) are key enzymes in lipid metabolism. Muscle and liver tissues are major main places. To study the role of the two enzymes during fasting, we detected the mRNA expression and activity changes of HSL and ACC in large yellow croaker muscle and liver tissues during 35 d fasting by real-time fluorescent quantitative PCR (qPCR) and Elisa, respectively. The results showed that fasting significantly decreased fat content and ACC mRNA level (P< 0.05), and increased HSL mRNA expression in both muscle and liver tissues (P<0.05). Moreover, fasting had a significant influence on the activities of HSL and ACC in both muscle and liver tissues of large yellow croaker (P<0.05). Muscle and liver HSL activities were moderately correlated with the corresponding mRNA expression level, as well as ACC in muscle. But liver ACC activity was negatively correlated with the mRNA level. These results suggested that the expression of HSL and ACC were regulated in both pre-translational and post-translational levels during fasting in the large yellow croaker. The results of the present study provide insights into the molecular mechanisms underlying the lipid metabolic response of the large yellow croaker to fasting.
Key words:    Larimichthys crocea    hormone sensitive lipase    acetyl-CoA carboxylase    fasting    mRNA expression    enzymatic activity   
Received: 2017-12-11   Revised: 2018-01-18
Tools
PDF (927 KB) Free
Print this page
Add to favorites
Authors
Articles by Qian Baoying
Articles by Huang Hongli
Articles by Li Juan
Articles by Cao Mingyue
Articles by Zhang Yu
Articles by Zhao Yayun
Articles by Xue Liangyi
References:
[1] Tian Juan, Tu Wei, Zeng Lingbing, et al. The changes in growth, serum biochemical indices and GH/IGF-Ⅰ/IN mRNA expression abundance of Orechromis niloticus during fasting and re-feeding[J]. Journal of Fisheries of China, 2012, 36(6):900-907.
[2] Jin Li, Zhao Na, Zhou Chuanjiang, et al. Effects of starvation on hematological parameters and hematogenesis in Myxocyprinus asiaticus[J]. Acta Hydrobiologica Sinica, 2012, 36(4):665-673.
[3] Qiao Zhigang, Zhang Jianping, Niu Jingyan, et al. Effects of starvation and refeeding on the blood indices of silurus asotus[J]. Acta Hydrobiologica Sinica, 2008, 32(5):631-636.
[4] Han Chunyan, Wen Xiaobo, Zheng Qingmei, et al. Effect of starvation on activities and mRNA expression of lipoprotein lipase and hormone-sensitive lipase in tilapia (Oreochromis niloticus×O. areus)[J]. Fish Physiology and Biochemistry, 2011, 37(1):113-122.
[5] Tian Juan, Wen Hua, Zeng Lingbing, et al. Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding[J]. Aquaculture, 2013, 400-401:29-35.
[6] Huang Hongli, Xue Liangyi, Wang Jun. Effects of fasting and re-feeding on the expression of Ⅰ-FABPb in Larimichthys crocea[J]. Chinese Journal of Cell Biology, 2016, 38(4):397-404.
[7] Lafontan M, Langin D. Lipolysis and lipid mobilization in human adipose tissue[J]. Progress in Lipid Research, 2009, 48(5):275-297.
[8] Ji Fuyun, Yu Qixing, Pan Peiwen. Chromosomal localization of the hormone-sensitive lipase gene (Hsl) in rice field eel[J]. Hereditas (Beijing), 2003, 25(2):163-167.
[9] Bertile F, Raclot T. ATGL and HSL are not coordinately regulated in response to fuel partitioning in fasted rats[J]. The Journal of Nutritional Biochemistry, 2011, 22(4):372-379.
[10] Chen Qiliang, Luo Zhi, Song Yufeng, et al. Hormone-sensitive lipase in yellow catfish Pelteobagrus fulvidraco:molecular characterization, mRNA tissue expression and transcriptional regulation by leptin in vivo and in vitro[J]. General and Comparative Endocrinology, 2014, 206:130-138.
[11] Zhang Yingjie, Liu Yueqin, Cheng Shanyan, et al. Effects of dietary energy level on the expression of the HSL gene in different tissues of sheep[J]. Journal of Integrative Agriculture, 2012, 11(7):1167-1172.
[12] Tang Zhiguo, Sun Caiyun, Yan Aifen, et al. Genes involved in fatty acid metabolism:molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides[J]. Molecular and Cellular Endocrinology, 2013, 376(1/2):114-124.
[13] Kim K W, Yamane H, Zondlo J, et al. Expression, purification, and characterization of human acetyl-CoA carboxylase 2[J]. Protein Expression and Purifcation, 2007, 53(1):16-23.
[14] Cheng Hanliang, Ji Nanjing, Peng Yongxing, et al. Molecular characterization and tissue-specific expression of the acetyl-CoA carboxylase α gene from Grass carp, Ctenopharyngodon idella[J]. Gene, 2011, 487(1):46-51.
[15] Najafpanah M J, Sadeghi M, Zali A, et al. Chromium downregulates the expression of Acetyl CoA Carboxylase 1 gene in lipogenic tissues of domestic goats:a potential strategy for meat quality improvement[J]. Gene, 2014, 543(2):253-258.
[16] Bengtsson C, Blaho S, Saitton D B, et al. Design of small molecule inhibitors of acetyl-CoA carboxylase 1 and 2 showing reduction of hepatic malonyl-CoA levels in vivo in obese Zucker rats[J]. Bioorganic & Medicinal Chemistry, 2011, 19(10):3039-3053.
[17] Sun Junjun, Lu Ronghua, Chang Zhiguang, et al. Effects of carbohydrates on transcription of five key genes involved in lipid metabolism in hepatopancreas of grass carp Ctenopharyngodon idella[J]. Fisheries Science, 2015, 34(11):696-701.
[18] Zhang Xiaqing, Xu Jianhe, Pan Qian, et al. Full length cDNA molecular cloning of acetyl-CoA carboxylase αgene and effects of dietary lipid level on its expression in the liver of half-smooth tongue sole (Cynoglossus semilaevis)[J]. Chinese Journal of Animal Nutrition, 2016, 28(2):485-497.
[19] Duan Qingyuan, Zhong Huiying, Si Liegang, et al. Comparative analyses of biochemical composition in net cultured and wild Pseudosciaena crocea (Richardson)[J]. Journal of Zhejiang Ocean University:Natural Science, 2000, 19(2):125-128.
[20] Zhang Xiaodong, Zhu Yongfei, Cai Lisheng, et al. Effects of fasting on the meat quality and antioxidant defenses of market-size farmed large yellow croaker (Pseudosciaena crocea)[J]. Aquaculture, 2008, 280(1/4):136-139.
[21] Johansson L, Kiessling A, Kiessling K H, et al. Effects of altered ration levels on sensory characteristics, lipid content and fatty acid composition of rainbow trout (Oncorhynchus mykiss)[J]. Food Quality and Preference, 2000, 11(3):247-254.
[22] Einen O, Thomassen M S. Starvation prior to slaughter in Atlantic salmon (Salmo salar) Ⅱ. White muscle composition and evaluation of freshness, texture and colour characteristics in raw and cooked fillets[J]. Aquaculture, 1998, 169(1/2):37-53.
[23] Rasmussen R S, Ostenfeld T H, McLean E. Growth and feed utilisation of rainbow trout subjected to changes in feed lipid concentrations[J]. Aquaculture International, 2000, 8(6):531-542.
[24] Zhang Chundan, Hu Yuzhen, Miao Liang, et al. Changes of fatty acid composition and content in Pseudosciaena crocea from net cage during the winter[J]. Journal of Marine Sciences, 2014, 32(2):80-84.
[25] Zheng Jialang, Zhu Qingling, Shen Bin, et al. Effects of starvation on lipid accumulation and antioxidant response in the right and left lobes of liver in large yellow croaker Pseudosciaena crocea[J]. Ecological Indicators, 2016, 66:269-274.
[26] Londos C, Brasaemle D L, Schultz C J, et al. On the control of lipolysis in adipocytes[J]. Annals of the New York Academy of Sciences, 1999, 892:155-168.
[27] Huang Hongli, Xue Liangyi, Shi Junxin, et al. Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker, Larimichthys crocea (Richardson), during fasting[J]. Aquaculture Research, 2017, 48(7):3493-3504.
[28] Sone H, Kamiyama S, Higuchi M, et al. Biotin augments acetyl CoA carboxylase 2 gene expression in the hypothalamus, leading to the suppression of food intake in mice[J]. Biochemical and Biophysical Research Communication, 2016, 476(3):134-139.
[29] Kinnunen S, Mänttäri S, Herzig K H, et al. Effects of wintertime fasting and seasonal adaptation on AMPK and ACC in hypothalamus, adipose tissue and liver of the raccoon dog (Nyctereutes procyonoides)[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 2016, 192:44-51.
[30] Pascual F, Carman G M. Phosphatidate phosphatase, a key regulator of lipid homeostasis[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2013, 1831(3):514-522.
Haiyang Xuebao